ABSTRACT
Background. Pneumonia is an acute infectious disease in which the respiratory sections of the lungs are affected with obligatory interalveolar exudation and infiltration of the parenchyma in response to the introduction of microorganisms. It is known that pneumonia is a clinical and radiological diagnosis. Chest CT scans are emerging as a useful tool in the diagnostic process of viral pneumonia cases associated with COVID-19. The CT manifestation of this viral pneumonia is non-specific but it has characteristic features based on which experienced radiologists can diagnose the disease. Findings are similar to other viral pneumonias but the localization of the signs is rather typical. Aim: To present the main radiological findings of various types of pneumonia and COVID-19 pneumonia. Methods. Generalized analysis of diagnosis of various types of pneumonia by the radiological method. Results. The study shows the main radiological signs of pneumonia, including Covid-19. A description scheme of chest radiography and an algorithm for diagnosing the main types of pneumonia were also shown. Conclusion. This article presents criteria for determining the severity of the condition of patients with coronavirus infection in accordance with radiological changes in the lung tissue.
ABSTRACT
Aim To investigate the inhibitory effect of copper gluconate and disulfiran on the PLpro of SARS-CoV-2 and to explore the effect of combination of them on PLpro.Methods SARS-CoV-2 PLpro was purified by recombinant expression technology of he effects of copper gluconate and disulfiram on PLpro activity were studied by enzyme kinetic method.Results Copper gluconate and disulfiram had high inhibitory activity on SARS-CoV-2 PLpro,Ubiquitin-7-Amino-4-methylcoumarin(Ub-AMC)was used as the fluorescent substrate.The IC50 of copper gluconate was 33.02 nmol.L-1,showing competitive inhibition,and the IC50 of disulfiram was 480.4 nmol.L-1,showing non competitive inhibition,and the combination of the two inhibitors showed the advantage of synergistic inhibition.Conclusions Copper gluconate and disulfiram have a high inhibitory effect on SARS-CoV-2 PLpro protein,and the combination shows the advantage of synergistic inhibition. Copyright © 2022 Publication Centre of Anhui Medical University. All rights reserved.